Saturday, April 18, 2020

Morphology and Ultra Structure of Bacteria MCQs

Microbiology Questions and Answers – Morphology and Fine Bacteria Structure – Size, Shape and Arrangement of Bacterial Cells.


1. In the classification of bacteria according to shape,which among the following refer to cuboidal arrangement of bacterial cells?
a) Tetrads
b) Staphylococci
c) Sarcinae
d) Streptococci
View Answer
Answer: c
Explanation: In Sarcinae group of bacteria, the cells divide in three planes, in a regular pattern, producing a cuboidal arrangement of cells. In Staphylococci, cells divide in three planes, in an irregular pattern,producing bunches of cocci.
2. Which group of bacteria among the following have the largest area of contact?
a) Palisades
b) Trichomes
c) Streptobacilli
d) Diplobacilli
View Answer
Answer: b
Explanation: Trichomes are similar to chains but have a much larger area of contact between the adjacent cells whereas in palisade arrangement, the cells are ind side by side at angles to one another.
3. Which among the following are “Spirochetes”?
a) Spirillum volutans
b) Corynebacterium diphtheriae
c) Streptomyces sp.
d) Treponema pallidum
View Answer
Answer: d
Explanation: Spirochetes are flexible and can twist and contort their shape, whereas spirilla are relatively rigid. Treponema palldium belong to the spirochetes group and Spirillum volutans belong to the spirilla group.
4. What is the approximate size of the bacterial cell?
a) 2mm in diameter
b) 1mm in diameter
c) 2 micrometer in diameter
d) 0.5 to 1.0 micrometer in diameter
View Answer
Answer: d
Explanation: Bacteria are very small, most being approximately 0.5 to 1.0 micrometer in diameter.This is the reason why they cannot be seen by naked eyes and can be observed under the microscope.
5. When rod shaped bacteria appears in pairs,it is known as?
a) Diplobacilli
b) Streptobacilli
c) Diplococci
d) Staphylococci
View Answer
Answer: a
Explanation: When bacilli occur in pairs, it is known as diplobacilli but when they form chains it is known as streptobacilli. Cocci are spherical shaped bacteria and not rod shaped.
6. Surface area/volume ratio of bacteria is exceedingly low compared to the same ratio for larger organisms of similar shape.
a) True
b) False
View Answer
Answer: b
Explanation: Due to the small size of microorganisms, the surface area/volume ratio of bacteria is exceedingly high compared to the same ratio for larger organisms of similar shape. A relatively large surface through which nutrients can enter ( or waste products leave) compared to a small volume of cell substance to be nourished accounts for the unusually high rate of growth and metabolism of bacteria.
7. Bacteria with less than a complete twist or comma shaped is known as?
a) spirilla
b) helical
c) vibrioid
d) spirochetes
View Answer
Answer: c
Explanation: Bacteria with less than a complete twist or turn have a vibrioid shape,whereas those with one or more complete turns have a helical shape. Spirilla are rigid helical bacteria whereas spirochetes are highly flexible.
8. Which of the following are functions of stalk?
a) nutrient absorption
b) motility
c) attachment of the cells to surfaces
d) human infection
View Answer
Answer: c
Explanation: Stalk are mainly nonliving ribbonlike or tubular appendages that are excreted by the cell. These stalks aid in attachment of the cells to surfaces.
1. Bacteria having cluster of flagella at both poles of cells are known as?
a) Lophotrichous
b) Peritrichous
c) Amphitrichous
d) Monotrichous
View Answer
Answer: c
Explanation: In amphitrichous, flagella occur either singly or in clusters at both cell poles. Lophotrichous refers to a cluster of polar flagella, peritrichous is surrounded by lateral flagella and monotrichous is for a single polar flagella.







Microbiology Questions and Answers – Morphology and Fine Bacteria – Structures External to the Cell Wall

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This set of Microbiology Multiple Choice Questions & Answers (MCQs) focuses on “Morphology and Fine Bacteria – Structures External to the Cell Wall”.
1. Bacteria having cluster of flagella at both poles of cells are known as?
a) Lophotrichous
b) Peritrichous
c) Amphitrichous
d) Monotrichous
View Answer
Answer: c
Explanation: In amphitrichous, flagella occur either singly or in clusters at both cell poles. Lophotrichous refers to a cluster of polar flagella, peritrichous is surrounded by lateral flagella and monotrichous is for a single polar flagella.
a) Amphitrichous
b) Peritrichous
c) Monotrichous
d) Lophotrichous
View Answer
Answer: b
Explanation: Salmonella typhi has peritrichous type of flagella arrangement. In peritrichous, the bacteria is surrounded by lateral flagella.
3. The L Ring in a Gram-Negative bacterium flagella is associated with_________
a) Peptidogycan
b) Outer Membrane
c) Cytoplasmic Membrane
d) Cell Membrane
View Answer
Answer: b
Explanation: The flagella in Gram-Negative bacteria has four basal rings. The L Ring of flagella is associated with the outer membrane. The P ring is associated with the peptidoglycan layer and both the M and S rings are associated with the cytoplasmic membrane.
4. Which among the following acts as a transport protein for protons in flagellar motion?
a) fli protein
b) cGMP
c) atp
d) mot protein
View Answer
Answer: d
Explanation: The proton binds to the Mot protein and this changes the conformation of Mot protein. It results in releasing the ring and helps in flagellar motion. The fli protein helps in changing the direction of flagella.
5. F pilus has a major role as___________
a) motility of the cell
b) port of entry of genetic material during mating
c) attachment to host cell
d) human infection
View Answer
Answer: b
Explanation: Pili are hollow,filamentous appendages that are thinner and shorter.They do not function in motility and have other different functions.One type known as F plus or sex plus serves as the port of entry of genetic material during bacterial mating.
6. Prosthecae helps in __________
a) motility
b) nutrient absorption and attachment to surfaces
c) human infection
d) protection from environment
View Answer
Answer: b
Explanation: Prosthecae increases the surface area of the cells for nutrient absorption, which is advantageous in dilute environments. Some prosthecate bacteria have an adhesive substance at the end of a prostheca that aids in attachment to surfaces.
7. The capsule of Klebsiella pneumoniae is composed of heteropolysaccharides.
a) True
b) False
View Answer
Answer: a
Explanation: Heteroploysaccharides are usually synthesized from sugar precursors that are activated within the cell, attached to a lipid carrier molecule, transported across the cytoplasmic membrane and polymerized outside the cell. The capsule of Klebsiella pneumoniae is an example of heteropolysaccharides.







Microbiology Questions and Answers – Morphology and Fine Bacteria – Structures External to the Cell Wall

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This set of Microbiology Multiple Choice Questions & Answers (MCQs) focuses on “Morphology and Fine Bacteria – Structures External to the Cell Wall”.
1. Bacteria having cluster of flagella at both poles of cells are known as?
a) Lophotrichous
b) Peritrichous
c) Amphitrichous
d) Monotrichous
View Answer
Answer: c
Explanation: In amphitrichous, flagella occur either singly or in clusters at both cell poles. Lophotrichous refers to a cluster of polar flagella, peritrichous is surrounded by lateral flagella and monotrichous is for a single polar flagella.

a) Amphitrichous
b) Peritrichous
c) Monotrichous
d) Lophotrichous
View Answer
Answer: b
Explanation: Salmonella typhi has peritrichous type of flagella arrangement. In peritrichous, the bacteria is surrounded by lateral flagella.
3. The L Ring in a Gram-Negative bacterium flagella is associated with_________
a) Peptidogycan
b) Outer Membrane
c) Cytoplasmic Membrane
d) Cell Membrane
View Answer
Answer: b
Explanation: The flagella in Gram-Negative bacteria has four basal rings. The L Ring of flagella is associated with the outer membrane. The P ring is associated with the peptidoglycan layer and both the M and S rings are associated with the cytoplasmic membrane.
4. Which among the following acts as a transport protein for protons in flagellar motion?
a) fli protein
b) cGMP
c) atp
d) mot protein
View Answer
Answer: d
Explanation: The proton binds to the Mot protein and this changes the conformation of Mot protein. It results in releasing the ring and helps in flagellar motion. The fli protein helps in changing the direction of flagella.
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5. F pilus has a major role as___________
a) motility of the cell
b) port of entry of genetic material during mating
c) attachment to host cell
d) human infection
View Answer
Answer: b
Explanation: Pili are hollow,filamentous appendages that are thinner and shorter.They do not function in motility and have other different functions.One type known as F plus or sex plus serves as the port of entry of genetic material during bacterial mating.
6. Prosthecae helps in __________
a) motility
b) nutrient absorption and attachment to surfaces
c) human infection
d) protection from environment
View Answer
Answer: b
Explanation: Prosthecae increases the surface area of the cells for nutrient absorption, which is advantageous in dilute environments. Some prosthecate bacteria have an adhesive substance at the end of a prostheca that aids in attachment to surfaces.
7. The capsule of Klebsiella pneumoniae is composed of heteropolysaccharides.
a) True
b) False
View Answer
Answer: a
Explanation: Heteroploysaccharides are usually synthesized from sugar precursors that are activated within the cell, attached to a lipid carrier molecule, transported across the cytoplasmic membrane and polymerized outside the cell. The capsule of Klebsiella pneumoniae is an example of heteropolysaccharids.
8. When a bacteria swim towards a chemical, it is termed as__________
a) positive chemotaxis
b) phototaxis
c) negative chemotaxis
d) magnetotaxis
View Answer
Answer: a
Explanation: When a bacteria swims towards or away from chemical compounds, the phenomenon is known as bacterial chemotaxis.Swimming towards a chemical is termed as positive chemotaxis; swimming away is negative chemotaxis.
9. Which type of force drives the flagellar motion?
a) Protonmotive force
b) ATP driven
c) Protonmotive and ATP driven
d) No protonmotive nor ATP driven
View Answer
Answer: a
Explanation: It is found that flagellar motor is driven by the protonmotive force, i.e., the force derived from the electrical potential and the hydrogen-ion gradient across the cytoplasmic membrane.

Microbiology Questions and Answers – Morphology and Fine Bacteria – Structures Internal to the Cell Wall


1. Polyisoprenoid branched-chain lipids, are present in which of the following?
a) Archaebacteria
b) Eubacteria
c) Archaebacteria and Eubacteria
d) Cannot be determined
View Answer
Answer: a
Explanation: In Archaebacteria,the lipids are polyisoprenoid branched-chain lipids, in which long-chain branched alcohols(phytanols) are ether linked to glycerol.In Eubacteria,the phospholipids are phosphoglycerides, in which straight-chain fatty acids are ester-linked to glycerol.







Microbiology Questions and Answers – Morphology and Fine Bacteria – Structures Internal to the Cell Wall

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This set of Microbiology Multiple Choice Questions & Answers (MCQs) focuses on “Morphology and Fine Bacteria – Structures Internal to the Cell Wall”.
1. Polyisoprenoid branched-chain lipids, are present in which of the following?
a) Archaebacteria
b) Eubacteria
c) Archaebacteria and Eubacteria
d) Cannot be determined
View Answer
Answer: a
Explanation: In Archaebacteria,the lipids are polyisoprenoid branched-chain lipids, in which long-chain branched alcohols(phytanols) are ether linked to glycerol.In Eubacteria,the phospholipids are phosphoglycerides, in which straight-chain fatty acids are ester-linked to glycerol.

2. Cytoplasmic membrane and the cell material bounded by it plus the outer membrane of cell is known as _________
a) Protoplast
b) Cytoplast
c) Spheroplast
d) Cell membrane
View Answer
Answer: c
Explanation: A protoplast is that portion of a bacterial cell consisting of the cytoplasmic membrane and the cell material bounded by it. When the cell has two membranes, the cytoplasmic membrane of the protoplast plus the outer membrane of the cell wall, the cell is called a spheroplast rather than protoplast.
3. Which among the following is associated with export of exocellular enzymes?
a) central mesosomes
b) peripheral mesosomes
c) thylakoids
d) nucleus
View Answer
Answer: b
Explanation: Peripheral mesosomes show only a shallow penetration into the cytoplasm, are not restricted to a central location, and are not associated with nuclear material and they are involved in export of exocellular enzymes such as penicillinase.
4. Ribosomes of prokaryotes have a sedimentation coefficient of?
a) 90S
b) 80S
c) 50S
d) 70S
View Answer
Answer: d
Explanation: Ribosomes of prokaryotes have a sedimentation coefficient of 70S and are composed of two subunits, a 50S and a 30S subunit. But the ribosomes of eukaryotes have a sedimentation coefficient of 80S and are composed of a 60S and a 40S subunit.
5.Poly-beta-hydroxybutyrate(PHB) present in aerobic bacteria can serve as?
a) a reserve carbon and energy source
b) a reserve source of phosphate
c) acceptor of oxygen
d) provides buoyancy
View Answer
Answer: a
Explanation: A polymer found in aerobic bacteria, especially under high-carbon,low-nitrogen culture conditions, is a chlorofoam-soluble, lipid like material, poly-beta-hydroxybutyrate(PHB), which can serve as a reserve carbon and energy source.







Microbiology Questions and Answers – Morphology and Fine Bacteria – Structures Internal to the Cell Wall

« 
 »

This set of Microbiology Multiple Choice Questions & Answers (MCQs) focuses on “Morphology and Fine Bacteria – Structures Internal to the Cell Wall”.
1. Polyisoprenoid branched-chain lipids, are present in which of the following?
a) Archaebacteria
b) Eubacteria
c) Archaebacteria and Eubacteria
d) Cannot be determined
View Answer
Answer: a
Explanation: In Archaebacteria,the lipids are polyisoprenoid branched-chain lipids, in which long-chain branched alcohols(phytanols) are ether linked to glycerol.In Eubacteria,the phospholipids are phosphoglycerides, in which straight-chain fatty acids are ester-linked to glycerol.
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2. Cytoplasmic membrane and the cell material bounded by it plus the outer membrane of cell is known as _________
a) Protoplast
b) Cytoplast
c) Spheroplast
d) Cell membrane
View Answer
Answer: c
Explanation: A protoplast is that portion of a bacterial cell consisting of the cytoplasmic membrane and the cell material bounded by it. When the cell has two membranes, the cytoplasmic membrane of the protoplast plus the outer membrane of the cell wall, the cell is called a spheroplast rather than protoplast.
3. Which among the following is associated with export of exocellular enzymes?
a) central mesosomes
b) peripheral mesosomes
c) thylakoids
d) nucleus
View Answer
Answer: b
Explanation: Peripheral mesosomes show only a shallow penetration into the cytoplasm, are not restricted to a central location, and are not associated with nuclear material and they are involved in export of exocellular enzymes such as penicillinase.
4. Ribosomes of prokaryotes have a sedimentation coefficient of?
a) 90S
b) 80S
c) 50S
d) 70S
View Answer
Answer: d
Explanation: Ribosomes of prokaryotes have a sedimentation coefficient of 70S and are composed of two subunits, a 50S and a 30S subunit. But the ribosomes of eukaryotes have a sedimentation coefficient of 80S and are composed of a 60S and a 40S subunit.
5.Poly-beta-hydroxybutyrate(PHB) present in aerobic bacteria can serve as?
a) a reserve carbon and energy source
b) a reserve source of phosphate
c) acceptor of oxygen
d) provides buoyancy
View Answer
Answer: a
Explanation: A polymer found in aerobic bacteria, especially under high-carbon,low-nitrogen culture conditions, is a chlorofoam-soluble, lipid like material, poly-beta-hydroxybutyrate(PHB), which can serve as a reserve carbon and energy source.

6. Which among the following compound when added to cytoplasmic membrane helps in maintaining the rigidity of cell?
a) lipopolysaccharide
b) hopanoid
c) phosphoglycerides
d) amino acids
View Answer
Answer: b
Explanation: Hopanoids are hydrophobic chemical compounds which when added to cytoplasmic membrane forms a stable and hard compound that helps in maintaining the rigidity of cell.
7. The nucleoid can be made visible under the light microscope by _________
a) Methylene blue
b) Iodine
c) Nile blue
d) Feulgen staining
View Answer
Answer: d
Explanation: Bacteria consist of nucleotide, consisting of a single, circular DNA molecule in which all the genes are linked and it is not a discrete nucleus. The nucleoid can be made visible under the light microscope by Feulgen staining,which is specific for DNA.
8. What helps in the heat resistance of endospore?
a) calcium-DPA complex
b) water
c) methylene
d) calcium
View Answer
Answer: a
Explanation: All endospores contain large amounts of dipicolinic acid (DPA). It occurs in combination with large amounts of calcium and is probably located in the central part of the spore.The calcium-DPA complex play a role in the heat resistance of endospores.
9. Cysts also have high heat resistance like endospores.
a) True
b) False
View Answer
Answer: b
Explanation: Cysts are dormant, thick-walled, desiccation-resistant forms that develop by differentiation of a vegetative cell and which can later germinate under suitable conditions. In some ways cysts resemble endospores but they do not have the high heat resistance of endospores.
10. Which of the following methods can be utilized for removing peripheral proteins of the cytoplasmic membrane?
a) treatment by detergents
b) osmotic shock
c) heat application
d) destruction of the membrane
View Answer
Answer: b
Explanation: Peripheral proteins are loosely attached to cytoplasmic membrane and can be removed by mild treatments such as osmotic shock. On the other hand, integral proteins can be removed only by destruction of the membrane, as with treatment by detergents.
11. Which of the following are true for cytoplasmic membrane?
a) hydrophilic barrier
b) hydrophobic barrier
c) site of generation of protonmotive force
d) hydrophobic barrier and site of generation of protonmotive force
View Answer
Answer: d
Explanation:The cytoplasmic membrane is a hydrophobic barrier to penetration by most water-soluble molecules. Because of its impermeability to protons, the cytoplasmic membrane is the site of generation of the proton motive force.

Tuesday, April 14, 2020

Lipids MCQs

                                    Lipids.                             

MCQ on Lipids and Fats (Biochemistry MCQ 009) with Answer Key.                                        (1). Which of the following molecule can act as molecular chaperons for assisting the folding of proteins?

a.      Carbohydratesb.      Vitaminsc.       Lipidsd.      Amides
(2). Which of the following macro-molecule can be most structurally diverse among living world?
a.      Carbohydratesb.      Proteinsc.       Nucleic acidsd.      Lipids
(3). Fat storing cells of vertebrates are called
a.      Hepatocytesb.      Asterocytesc.       Adipocytesd.      Melanocytes
(4). The enzyme abundantly distributed in adipocytes and germinating seeds are
a.      Proteasesb.      Lipasec.       Cellulased.      Nuclease.                                                (5). Rancidity of lipids of lipid rich food stuffs is due to: a                 Hydrogenation of unsaturated fatty acids
b.      Reduction of fatty acidsc.       Oxidation of fatty acidsd.      Dehydrogenation of saturation fatty acids
(6). Main function of preen glands in birds is
a.      Hormone secretionb.      Fatty acid degradationc.       Wax secretiond.      Pigment synthesis
(7). Which of the following statement is true
a.      Oxidative rancidity is observed more frequently in animal fats than vegetable fatsb.      Oxidative rancidity is observed more frequently in vegetable fats than animal fatsc.       Plants fats ado not undergo oxidative rancidityd.      Oxidative rancidity can be effectively checked by dehydrogenation of fatty acids
(8). Number of milligrams of KOH required to neutralize fatty acid present in 1 g of fat is called
a.      Potassium numberb.      Acid numberc.       Saponification numberd.      Iodine number
(9). Saponification number is the number of milligrams of KOH required to saponify 1 g fat. Which of the following statement is true about saponification number?
a.       The shorter is the chain length of fatty acids the higher is the saponification numberb.      The shorter is the chain length of fatty acids, the lower will be the saponification numberc.       The higher the chain saturation of fatty acid, the lower will be saponification numberd.      The lower is the saturation of fatty acid, the higher will be saponification number
(10).  The degree of unsaturation of lipid can be measured as ____________.a.      Saponification number
b.      Iodine numberc.       Polenske numberd.      Reichert Meissil Number
(11).  The number of OH group in fats can be expressed as _____________.
a.      Polenske numberb.      Reichert-Meissil numberc.       Acetyl numberd.      Iodine number
(12).  Polenske value of fatty acid indicates __________.
a.      How much unsaturation is there in the fatty acidb.      Level of saturation in the fatty acidc.       Indication of branching of fatty acid in the fatd.      How much volatile fatty acid can be extracted through saponification
(13).  Which of the following is an example for derived lipids?
a.      Steroidsb.      Terpenesc.       Carotenoidsd.      All of these
(14).  Naturally occurring fats are ______
a.      L typesb.      D typesc.       An equi-molar mixture of L and D typesd.      Symmetric
(15).  Generally fats with unsaturated fatty acids are ____________ at room temperature (25oC)
a.      Solidb.      Liquidc.       Liquid in the presence of oxygend.      Liquid in the absence of oxygen


MCQ on Lipids and Fats (Biochemistry MCQ 009) with Answer Key


mcq biology


Fat triglyceride shorthand formula
An Unsaturated Fat (source wikipedia)
MCQ on Lipids, Fats & WaxesBiochemistry MCQ 008(Multiple Choice Questions in Biology / Life Science)(Sample/Model/Practice Questions for JRF/NET Life Science Examination, ICMR JRF, DBT BET JRF, GATE, ICAR NET, PG Entrance)

(1). Which of the following molecule can act as molecular chaperons for assisting the folding of proteins?
a.      Carbohydratesb.      Vitaminsc.       Lipidsd.      Amides
(2). Which of the following macro-molecule can be most structurally diverse among living world?
a.      Carbohydratesb.      Proteinsc.       Nucleic acidsd.      Lipids
(3). Fat storing cells of vertebrates are called
a.      Hepatocytesb.      Asterocytesc.       Adipocytesd.      Melanocytes
(4). The enzyme abundantly distributed in adipocytes and germinating seeds are
a.      Proteasesb.      Lipasec.       Cellulased.      Nuclease
Blausen 0012 AdiposeTissue
(Source wikidpedia)
(5). Rancidity of lipids of lipid rich food stuffs is due to:

a.      Hydrogenation of unsaturated fatty acidsb.      Reduction of fatty acidsc.       Oxidation of fatty acidsd.      Dehydrogenation of saturation fatty acids
(6). Main function of preen glands in birds is
a.      Hormone secretionb.      Fatty acid degradationc.       Wax secretiond.      Pigment synthesis
(7). Which of the following statement is true
a.      Oxidative rancidity is observed more frequently in animal fats than vegetable fatsb.      Oxidative rancidity is observed more frequently in vegetable fats than animal fatsc.       Plants fats ado not undergo oxidative rancidityd.      Oxidative rancidity can be effectively checked by dehydrogenation of fatty acids
(8). Number of milligrams of KOH required to neutralize fatty acid present in 1 g of fat is called
a.      Potassium numberb.      Acid numberc.       Saponification numberd.      Iodine number
(9). Saponification number is the number of milligrams of KOH required to saponify 1 g fat. Which of the following statement is true about saponification number?
a.       The shorter is the chain length of fatty acids the higher is the saponification numberb.      The shorter is the chain length of fatty acids, the lower will be the saponification numberc.       The higher the chain saturation of fatty acid, the lower will be saponification numberd.      The lower is the saturation of fatty acid, the higher will be saponification number
(10).  The degree of unsaturation of lipid can be measured as ____________.

a.      Saponification numberb.      Iodine numberc.       Polenske numberd.      Reichert Meissil Number
(11).  The number of OH group in fats can be expressed as _____________.
a.      Polenske numberb.      Reichert-Meissil numberc.       Acetyl numberd.      Iodine number
(12).  Polenske value of fatty acid indicates __________.
a.      How much unsaturation is there in the fatty acidb.      Level of saturation in the fatty acidc.       Indication of branching of fatty acid in the fatd.      How much volatile fatty acid can be extracted through saponification
(13).  Which of the following is an example for derived lipids?
a.      Steroidsb.      Terpenesc.       Carotenoidsd.      All of these
(14).  Naturally occurring fats are ______
a.      L typesb.      D typesc.       An equi-molar mixture of L and D typesd.      Symmetric
(15).  Generally fats with unsaturated fatty acids are ____________ at room temperature (25oC)
a.      Solidb.      Liquidc.       Liquid in the presence of oxygend.      Liquid in the absence of oxygen
Honey comb

(16).  Carnauba wax is an example for __________
a.      Liquid waxb.      Soft waxc.       Hard waxd.      Archaebacterial wax
(17).  Specific gravity of lipid is________.
a.      0.2b.      0.8c.       1.0d.      1.5
(18).  Greater the number of carbon atom in chain of fatty acid __________a.      The boiling point will be higher
b.      The boiling point will be lesserc.       The melting point will be higherd.      The melting point will be lower

(19).  Dietary fats are transported as
a.      Chylomicronsb.      Liposomesc.       Lipid globulesd.      Oil droplets
(20).  Beta-oxidation of fatty acids takes place at
a.      Peroxisomeb.      Mitochondriac.       Mitochondria and Peroxisomed.      Mitochondria, Peroxisome and ER

Answer key and explanation
1. Ans. (c). Lipids
Chaperons are the biomolecules which assists the folding of proteins. Proteins are the common chaperons in the cells. Very rarely some lipids also acts as molecular chaperon
2. Ans. (d). Lipids
3. Ans. (c). Adipocytes
There are two types of adipocytes: white adipocytes which store white fat and brown adipocytes which stores brown fat. White adipocytes are large and univacuolar containing a single large droplet of lipid surrounded by a thin layer of cytoplasm. The brown adipocytes are plurivauolar containing many lipid droplets and plenty of cytoplasm.
4. Ans. (b). LipaseLipase enzyme hydrolyzes the ester bond between glycerol and fatty acid in a fat (lipid) and yield free fatty acids and glycerol. The liberated fatty acid can enter in the beta-oxidation and can yield Acetyl Co-A. Acetyl Co-A can enter into Kreb’s cycle and can yield ATP (energy). Germinating seeds and adipocytes contains fat as reserved food materials.
Human Pancreatic Lipase (HPL) is the lipase enzyme which catalyze the hydrolyze of dietary lipids.
5. Ans. (c). Oxidation of fatty acids
Oxidative breakdown of fats (fatty acids) by the atmospheric oxygen cause rancidity of lipids. The oxidation of lipids generates volatile short chain aldehydes and ketones which have very offensive smell.
6. Ans. (c). Wax secretion
Preen gland is also called as uropygial gland or oil glands, which are found in birds. Preen glands secrete oil called preen oil which is used to coat over the feathers, beaks and body parts.
7. Ans. (a). Oxidative rancidity is observed more frequently in animal fats than vegetable fats
8. Ans. (b). Acid number
9. Ans. (a). The shorter is the chain length of fatty acids the higher is the saponification number
10.  Ans. (b). Iodine number
11.  Ans. (c). Acetyl number
12.  Ans. (d). How much volatile fatty acid can be extracted through saponification
13.  Ans. (d). All of these

Derived lipids are lipids derived from simple or compound lipids by hydrolysis.
14.  Ans. (a) L-type
15.  Ans. (b). Liquid
Unsaturation of fatty acid cause a kink in the chain which disturb the compact packing of molecules and thus fats containing unsaturated fatty acids will be liquid at room temperature
16.  Ans. (c). Hard wax
Carnauba wax is also called as Brazil wax and palm wax, is a wax obtained from a palm namely Copernicia prunifera
17.  Ans. (b). 0.8
Density of water is 1 (exactly 0.9982). Since the density of fat is less than water, fats float over the water.
18.  Ans. (c). The melting point will be higher
19.  Ans. (a). Chylomicrons
20.  Ans. (b). Mitochondria

MCQ on Biochemistry - Lipids

Classification of lipids
1.  Fats are abundantly found in
a) Reproductive tissue
b) Vegetative tissue
c) Both a and b
d) None of these

2. Natural lipids are readily soluble in
a) Oil
b) Mercury
c) Water
d) None of these

3. Which of the following is/are unsaturated fatty acids?
a) Linoleic acid
b) Oleic acid
c) Palmitoleic acid
d) All of these

4. Fats can be stored in the cell without disturbing their
a) Hygroxyl bond
b)Aleurone layer
c) Osmotic relations
d) None of these

5. Liquid form of triglycerides at ordinary room temperature are called
a) Oils
b)Solid
c) Fats
d) None of these

6. The synthesis of glucose from fat is called
a) Glycolysis
b)Krebs cycle
c) Saponification
d) Gluconeogenesis

7. Hydrolysis of fats by alkalies into fatty acids and glycerol is called
a) Coagulation
b) Saponification
c) Suspension
d) Colloidal

8. The fats and oils are respectively rich in
a) Unsaturated fatty acids
b) Saturated fatty acids
c) Saturated and unsaturated fatty acids
d) None of these

9. β-oxidation takes place in
a) Mitochondria
b) cytoplasm
c) Chloroplasts
d) nucleus

10.  Which is a phospholipid
a) Lecithin
b) Cholesterol
c) Sterol
d) Steroid

11. The number of double bonds in Arachidonic acid
a) 1
b) 2
c) 3
d) 4

12. Essential fatty acids are
a) Linoleic acid
b) Arachidonic acid
c) Linolenic acid
d) All of these

13.  The following is not a phospholipid
a) Sphingomyelin
b) Lecithin
c) Cephalin
d) Cerebroside

14. Examples of monounsaturated fatty acids are:
a) Oleic acid
b) Arachidonic acid
c) Palmitic acid
d) Linolenic acid

15. High content of Triglycerides are seen in
a) LDL
b) HDL
c) VLDL
d) Chylomicrons

Answers:
1- a
2-d
3-d
4-c
5-a
6-d
7-b
8-c
9-a
10-a
11-d
12-d
13-d
14-a
15-d